Code: NED0060
Silica membrane spin column for DNA viruses and bacteria / direct samples
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Packaging configuration:
NED0060L 50 preparations
NED0061L 50 preparations DiaExtractR DNA Mini Kit
INTENDED USE
DiaExtract DNA Mini kit is applied in laboratories for the preparation of deoxyribonucleic acids from sample of interest and it can be used in case of microbial analysis for DNA extraction from low CFU containing samples. For molecular identification methods, like PCR, DNAs are required in high quality and sufficient amount. The DiaExtract DNA Mini kit is an optimal preparation system for fast and reliable isolation of DNAs from samples. The isolated DNA is ideal and ready-to-use in PCR reaction.
TEST PRINCIPLE
This kit performs quick isolation of DNA from human samples. The system is designed for use in traditional enzyme and lysis buffer-mediated lysis of human samples. The traditional way for lysing the samples is advised, supplementing the starting Lysis Buffer with lysosime, Proteinase K or Protease. Following lysis, by the help of Spin Column, DNA of the supernatant will be bound selectively. DNA is then purified and desalinated with Inhibitor Removal Buffer and Wash Buffer. Finally, the eluted DNA is ready for digestion, electrophoresis, PCR, or any other molecular applications.
KIT SPECIFICATION
The isolation system is specific for DNA from human samples taken directly or after pre-enrichment. The purity and concentration of isolated DNA make it highly recommended for further molecular biological protocols like PCR, enzymatic digestions, electrophoresis, etc. For efficient isolation and subsequent applications, the use of carrier nucleic acid at a final concentration of 1 g/l is required.