Code: NAA0200
Aspecific amplification with PCR or real-time PCR method
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Packagingconfigurations:
NAA0200L 50 reactions
NAA0200C 100 reactions
INTENDED USE
This kit is an aspecific kit and applied for amplification process (PCR, real-time PCR). The user has to provide the specific primers (and specific fluorescence marked probes in case of real-time PCR reaction) that necessary to the detection of the genetic segment which is wanted to be determined. The DiaTaq kit contains all the required component for the construction of the successful amplification. The user has to take into account the directives of own specific primers (and fluorescent marked probes) before optimize the reaction. This process is conduced by MgCl2 solution - the required amount can be measure into the reaction mix - which is part of the kit. DNA or cDNA can be template of the amplification.
TEST PRINCIPLE
Both steps of PCR reaction happen at determined temperature. The first step of PCR is the starting denaturation of DNA, the second step is the amplification which is regulated by primers. During every cycle of amplification happen the denaturation-annealing-elongation process. The product of PCR cycles is the multiplyed DNA. The PCR reaction is regulated by users specific primer-probe oligonucleotid pairs.