Diadifton Argos/Pentra

Code: 20301P
Diadifton Argos/Pentra

INTENDED USE
(For In Vitro Diagnostic Use Only)
Diadifton Argos/Pentra is used by ABX Pentra 60, 80, 120, Coulter AcT 5Diff hematological analyzer as a whole blood sample DILUTING reagent.

SUMMARY AND PRINCIPLE
FOR PENTRA 60,80, Coulter AcT 5 Diff: A sample volume of a whole blood specimen is aspirated into the analyzer where a portion of it is automatically diluted with Diadifton Argos/Pentra. A portion of this first dilution is further diluted with Diadifton Argos/Pentra. This second dilution of the sample is then introduced into impedance particle analyzer where the red blood cell count (RBC) and the thrombocyte count (PLT) is measured. To the remainder of the first dilution of the RBC sample a hemoglobin lyse reagent (Dialyse-Argos Pentra) is added for hemoglobin (HGB) measurement. A second portion of the aspirated blood sample is also diluted with lyse reagent (Diafix EO5 Diff. P60) and diluent is added at the end of the reaction as a stop reagent. From this aliquot the flow cytometer measures white blood cell count (WBC), lymphocyte count (LYM), monocyte cell count (MONO), eosinophil cell count (EO), neutrophil cell count (NEU). A third portion of the aspirated blood sample is lysed with a lysing reagent (Dialyse-Baso II) for basophil cell count (BASO) measurement in an impedance detector.  FOR PENTRA 120: A sample volume of a whole blood specimen is aspirated into the analyzer where a portion of it is automatically diluted with Diadifton Argos/Pentra. This dilution of the sample is then introduced into impedance particle analyzer where the red blood cell count (RBC) and the thrombocyte count (PLT) is measured. Another portion of the blood sample is also diluted and a hemoglobin lyse reagent (Dialyse-Argos Pentra) is added for hemoglobin (HGB) photometric and white blood cell (WBC) impedance measurements.A thrid portion of the aspirated blood sample is also diluted with lyse reagent (Diafix EO 5 Diff. P120) and diluent is added at the end of the reaction as a stop reagent. From this aliquot the flow cytometer measures white blood cell count (WBC), lymphocyte count (LYM), monocyte cell count (MONO), eosinophil cell count (EO), neutrophil cell count (NEU). A fourth portion of the aspirated blood sample is lysed with a WBC lysing reagent (Dialyse-Baso 5 Diff) for basophil cell count (BASO) measurement in an impedance detector.