INTENDED USE
(For In Vitro Diagnostic Use Only)
Diastromlyser-SYS-FBA is used by the Sysmex SF-, XT-, XE- series hematological analyzers red blood cell hemolysing reagent for the assay of white blood cell count, basophil.
For further information see the valid Instruction of use (IFU)
SUMMARY AND PRINCIPLE
A sample volume of a whole blood specimen is aspirated into the analyzer where a portion of it is automatically diluted with Diastromlyser-SYS-FBA. The red blood cells are hemolysed and all white blood cells, except basophil cells, loose most of their cytoplasm and shrink in size. From the Baso reaction chamber the cell suspension flows through the flowcell along with Diaton-SYS Diluent. The two fluids flow as independent, concentric streams. Tthe Diaton-SYS Diluent stream is encasing the sample stream. The sample is then analyzed by two-angle laser light scattering detection using a laser diode. The cells in the suspension are classified into red blood ghosts, basophils and all the other white blood cells. Consult your specific instrument Operators Manual for additional information with respect to procedures and principles for whole blood hematological analysis.