Code: 24117
|
|
INTENDED USE
(For In Vitro Diagnostic Use Only)
Perox Sheath is used by Technicon H*1,H*2,H*3 hematological analyzer as a whole blood sample SHEATH reagent.
SUMMARY AND PRINCIPLE
The peroxidase cytochemical reaction consists of 2 steps. In the first step, EDTA anticoagulated whole-blood sample is diluted with Perox Dil. 1 reagent. Surfactants and thermal stress cause lysis of the red blood cells. An aldehyde in Perox Dil. 1 reagent fixes the white blood cells. During the second step, Perox Di.l 2 reagent and Perox Dil. 3 reagent are added to the peroxidase reaction chamber. A peroxidase enzyme substrate in Perox Dil. 2 reagent and the hydrogen peroxide in Perox Dil. 3 reagent form a dark precipitate in white blood cells. A constant volume of the cell suspension from the Perox reaction chamber passes through the flowcell along with Perox Sheath. The two fluids flow as independent, concentric streams (no mixing), with the Perox Sheath stream encasing the sample stream.